Journal
ANALYTICAL BIOCHEMISTRY
Volume 414, Issue 2, Pages 196-201Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ab.2011.03.019
Keywords
Label-free; Immunosenor; Human serum chorionic gonadotropin; Nanoporous gold; Graphene sheets
Funding
- Natural Science Foundation of China [21075052]
- Natural Science Foundation of Shandong Province [Y2008B44]
- Key Subject Research Foundation of Shandong Province [XTD0705]
- Science and Technology Key Plan Project of Shandong Province [2010GSF10628]
- Science and Technology Development Plan Project of Jinan City [201004015]
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A label-free amperometric immunosensor for fast and sensitive assay of human serum chorionic gonadotropin (hCG) is presented. hCG was immobilized on nanoporous gold (NPG) foils and using hydroquinone (HQ) redox species as indicator. The variation of amperometric response to the concentration of hCG, the target antigen, was evaluated by cyclic voltammetry in phosphate-buffered solution. Taking advantage of dual-amplification effects of the NPG foils and graphene sheets (GSs), the immunosensor exhibited a specific response to hCG in the range of 0.5-40.00 ng ml(-1) with a detection limit of 0.034 ng ml(-1) under optimal conditions. It was demonstrated that our proposed method possesses good accuracy, acceptable precision, and reproducibility. The NPG showed a better sensitizing effect and stability as immobilization matrices. Crown Copyright (C) 2011 Published by Elsevier Inc. All rights reserved.
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