Journal
ANALYTICAL BIOCHEMISTRY
Volume 416, Issue 2, Pages 237-239Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ab.2011.04.040
Keywords
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Funding
- Ministry of Education, Culture, Sports, Science, and Technology (MEXT), Japan [20700368]
- Grants-in-Aid for Scientific Research [19101008, 20700368, 23390191] Funding Source: KAKEN
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Because construction of expression vectors is the first requisite in the functional analysis of genes, development of simple cloning systems is a major requirement during the postgenomic era. In the current study, we developed cloning vectors for gain- or loss-of-function studies by using the GFPuv gene as a positive/negative indicator of cloning. These vectors allow us to easily detect correct clones and obtain expression vectors from a simple procedure by means of the combined use of the GFPuv gene and a type IIS restriction enzyme. (C) 2011 Elsevier Inc. All rights reserved.
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