Journal
ANALYTICAL BIOCHEMISTRY
Volume 416, Issue 1, Pages 123-125Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ab.2011.05.009
Keywords
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Funding
- Wellbeing of Women [239281]
- Yorkshire Cancer Research [516898]
- Wellbeing of Women [RG1210] Funding Source: researchfish
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Laser capture microdissection of frozen tissue sections allows homogeneous cell populations to be isolated for expression profiling. However, this requires striking a balance between retaining adequate morphology for accurate microdissection and maintaining RNA integrity. Various staining protocols were applied to frozen endometrial carcinoma tissue sections. Although alcohol-based methods were superior to aqueous stains for maintaining RNA integrity, they suffered from irreproducible staining intensity. We developed a modified alcohol-based, buffered cresyl violet staining protocol that provides reproducible staining with minimal RNA degradation suitable for tissues with moderate to high levels of intrinsic RNase activity. (C) 2011 Elsevier Inc. All rights reserved.
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