Journal
ANALYTICAL BIOCHEMISTRY
Volume 412, Issue 1, Pages 114-116Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ab.2010.12.030
Keywords
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Funding
- Natural Science Foundation of China [20636010, 20820102036, 20906095]
- National Basic Research Development Program of China [2007CB714305]
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Staphylokinase (SAK) is a therapeutic protein with promise for thrombolytic therapy of acute myocardial infarction. In this study, polyethylene glycol (PEG) aldehyde was used for N-terminal PEGylation of SAK to improve the pharmacological profiles of SAK. Due to the presence of the competitive PEGylation between the N terminus and the Lys residues, kinetic and stoichiometric analysis was carried out to investigate the process for the N-terminal PEGylation of SAK. To achieve this objective, size exclusion chromatography and tryptic peptide mapping were used to measure the PEGylation extent of SAIL molecule and its specific amino acid residues, respectively. (C) 2010 Elsevier Inc. All rights reserved.
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