Journal
ANALYTICAL BIOCHEMISTRY
Volume 419, Issue 2, Pages 211-216Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ab.2011.08.011
Keywords
Br signature; De novo sequencing; C-terminal; Derivatization; Oxazolone
Funding
- BK21 Program
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Due to almost identical chemical properties of C-terminal and side-chain carboxylic groups, selective C-terminal derivatization has been difficult. Although oxazolone-based C-terminal derivatization is the only selective C-terminal modification available, it has not been used widely because of its low derivatization efficiency. In this paper, an improved oxazolone chemistry for incorporation of Br signature to C-terminus is reported. MS/MS analysis of the brominated peptides led to a series of y ions with Br signature, facilitating de novo C-terminal sequencing. (C) 2011 Elsevier Inc. All rights reserved.
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