Preparation and properties of highly stable innocuous niosome in Span 80/PEG 400/H2O system

The highly stable innocuous niosome composed of only three components is successfully prepared in Span 80/PEG 400/H2O system. The niosome properties are studied by some means of freeze fracture replication-transmission electron microscopy, negative staining-transmission electron microscopy, dynamic light scattering and differential scanning calorimetry. The obtained results indicate that the niosome can be stable for over one year. The niosome diameter is between 100 and 180 nm. The compositions of the system affect the preparation and properties of the niosome. But the temperature and ionic intensity do not distinctly change the stability and the radius. The formation mechanism of the niosome is discussed in this paper. (C) 2007 Elsevier B.V. All rights reserved.