Journal
ANALYTICAL BIOCHEMISTRY
Volume 407, Issue 1, Pages 147-149Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ab.2010.08.004
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Funding
- NCI NIH HHS [R03CA142075-01, R03 CA142075-01, R03 CA142075] Funding Source: Medline
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We observed the preservation of microRNAs in unrefrigerated dried serum blots. Preservation was not adversely affected by drying or storing at 37, 45, or 60 degrees C instead of room temperature, but it was harmed when blots were dried incompletely before storage. Preservation of microRNAs in serum was not diminished if, instead of being kept frozen at -80 degrees C, it was stored as dried blots at room temperature for 5 months or at 37 degrees C for 4 weeks. Thus, dried blots can be a convenient and safer way to save, transport, and store serum for microRNA assays. Published by Elsevier Inc.
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