Journal
ANALYTICAL BIOCHEMISTRY
Volume 404, Issue 2, Pages 135-139Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ab.2010.04.038
Keywords
Calcein; Fluorescence spectroscopy; Histamine; Ligand exchange; Sensor molecule
Funding
- Ministry of Education, Culture, Science, Sports, and Technology of Japan
- Japan Society for the Promotion of Science (JSPS)
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We report on a novel histamine monitoring method by using a fluorescent probe, a complex between Ni2+ and calcein, based on a ligand exchange mechanism. The fluorescence intensity of this probe, which has been reduced due to effective quenching by Ni2+. ion, increases drastically by an addition of histamine. Furthermore, the probe shows high selectivity toward histamine among the various neurotransmitters in 0.1 M phosphate buffer solution (pH 7.4). Biomonitoring studies to detect histamine released from RAW264 cells are successfully represented. (C) 2010 Elsevier Inc. All rights reserved.
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