Journal
ANALYTICAL BIOCHEMISTRY
Volume 405, Issue 2, Pages 230-235Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ab.2010.06.014
Keywords
Polyamidoamine dendrimer (PAMAM); Immobilized capillary enzyme microreactor; Glucose oxidase (GOx); beta-D-Glucose; Gluconic acid; Capillary zone electrophoresis
Funding
- National Natural Science Foundation of China [20375004, 90713013]
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Polyamidoamine dendrimer (PAMAM) is one of a number of dendritic polymers with precise molecular structure, highly geometric symmetry, and a large number of terminal groups. In this study, different generations of PAMAM (G0-G4) were introduced onto the inner wall of fused-silica capillaries by microwave irradiation and a new type of glucose oxidase (GOx) capillary enzyme microreactor was developed based on enzyme immobilization in the prepared PAMAM-grafted fused-silica capillaries. The optimal enzymolysis conditions for beta-D-glucose in the microreactor were evaluated by capillary zone electrophoresis. In addition, the enzymolysis efficiencies of different generations of PAMAM-GOx capillary enzyme microreactor were compared. The results indicate that enzymolysis efficiency increased with increasing generations of PAMAM. The experimental results provide the possibility for the development and application of an online immobilized capillary enzyme microreactor. Crown Copyright (C) 2010 Published by Elsevier Inc. All rights reserved.
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