Journal
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
Volume 129, Issue 29, Pages 8966-+Publisher
AMER CHEMICAL SOC
DOI: 10.1021/ja0735709
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Funding
- Howard Hughes Medical Institute Funding Source: Medline
- NCI NIH HHS [U54 CA119341-02, U54 CA119341] Funding Source: Medline
- NIGMS NIH HHS [T32 GM008720-01, T32 GM008720] Funding Source: Medline
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This paper reports a strategy that uses microfluidic networks to pattern self-assembled monolayers with gradient microislands for the attachment of individual cells. A microfluidic network is first used to pattern a monolayer into square regions that present maleimide groups and then used to flow a solution having a gradient of the cell adhesion peptide Arg-Gly-Asp over the substrate. In this way, the surface is patterned with microislands approximately 33 x 33 micrometers in size and each having a defined gradient of immobilized cell adhesion ligand. B16F10 cells were allowed to attach to the patterned islands and were found to display a nonuniform distribution of cytoskeletal structures in response to the gradient of adhesion ligand. This work is significant because it permits studies of the influence of a nonuniform microenvironment on the polarization, differentiation, and signaling of adherent cells.
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