Serotonin 5-HT2C receptor homodimerization is not regulated by agonist or inverse agonist treatment

Serotonin 5-HT2C receptors represent targets for therapeutics aimed at treating anxiety, depression, schizophrenia, and obesity. Previously, we demonstrated that 5-HT2C receptors function as homodimers. Herein, we investigated the effect of agonist and inverse agonist treatment on the homodimer status of two naturally occurring 5-HT2C receptor isciforms, one without basal activity (VGV) and one with constitutive activity (INI) with respect to G(alpha q) signaling. Cyan- and yellow-fluorescent proteins were used to monitor VGV and IN I homodimer formation by western blot, and in living cells using bioluminescence and fluorescence resonance energy transfer (BRET and FRET). Western blots of solubilized membrane proteins revealed equal proportions of homodimeric receptor species from HEK293 cells transfected with either the VGV or INI isoform in the absence and presence of 5-HT. BRET ratios measured in HEK293 cells transfected with the VGV or INI isoform were the same and were not modulated by 5-HT. Similarly, FRET efficiencies were the same regardless of whether measured in cells expressing the VGV or INI isoform in the absence or presence of 5-HT or clozapine. The results indicate that serotonin 5-HT2C receptors form homodimers regardless of whether they are in an inactive or active conformation and are not regulated by drug treatment. (C) 2007 Elsevier B.V. All rights reserved.