Journal
ANALYTICAL BIOCHEMISTRY
Volume 391, Issue 1, Pages 64-68Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ab.2009.04.034
Keywords
Saliva; Microbiome diversity; Barcoded pyrosequencing; Cloning
Funding
- Max Planck Society
Ask authors/readers for more resources
Metagenomic studies traditionally rely on cloning polymerase chain reaction (PCR) products and sequencing multiple clones. However, this approach is tedious and expensive, thereby limiting the range and scale of questions that can be addressed. Recent developments in DNA sequencing technologies enable a dramatic increase in throughput via parallel in-depth analysis of many samples with limited sample processing and lower costs. We directly compared the traditional cloning approach with a barcoded pyrosequencing method to see whether the latter accurately describes microbiome diversity in human saliva. Our results indicate that despite the shorter read lengths, the pyrosequencing approach Provides a description of the human salivary microbiome that is in good agreement with results based on the traditional cloning and sequencing approach. (C) 2009 Elsevier Inc. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available