Journal
ANALYTICAL BIOCHEMISTRY
Volume 391, Issue 2, Pages 151-153Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ab.2009.05.015
Keywords
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Funding
- Ministry of Education, Science, and Technology [M10417060004-04N1706-00410]
- Korea government (MOST) [R0A-2007-000-10007-0]
- National Research Foundation of Korea [R0A-2007-000-10007-0] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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We present a rapid and high-throughput human serum N-glycan preparation technology using 96-well plate-based procedures, The released N-glycans front polyvinylidene fluoride (PVDF) membrane filter plate are subsequently loaded to porous graphitic carbon (PGC) containing a 96-well plate to remove salts and other contaminants without sacrificing accuracy or reproducibility. This robust glycan Preparation technology is applied to ovarian cancer diagnosis using 5 mu l of patient Serum. (C) 2009 Elsevier Inc. All rights reserved.
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