Journal
ANALYTICAL BIOCHEMISTRY
Volume 389, Issue 1, Pages 32-39Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ab.2009.03.020
Keywords
Carbofuran; Triazophos; Colloidal gold; Lateral-flow strip assay; Bispecific monoclonal antibody
Funding
- National Natural Science Foundation of China [30671391]
- Important Technology Special Topic Project of Zhejiang Province, China [2006C12102]
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Using a simple test for rapid identification and quantification of pesticide multiresidues in food and environmental samples is a long-cherished approach for practical monitoring purposes. Here two gold-based lateral-flow strips (strip A and Strip B) were investigated for simultaneous detection of carbofuran and triazophos. For the strip A format, a bispecific monoclonal antibody (BsMcAb) against both carbofuran and triazophos was employed to prepare the immunogold probe. For the strip B format, anti-carbofuran monoclonal antibody (McAb) and anti-triazophos McAb separately labeled with colloidal gold were combined as detector reagents. By comparison of visual results from pesticide standard tests between the two formats, the strip B assay manifested higher sensitivities for both pesticides. Analysis of spiked water samples by the preferable strip indicated that the detection limits for carbofuran and triazophos were 32 and 4 mu g/L, respectively. The strength of the portable one-step strip assay was In the Simultaneous screening for two pesticides within a short time (8-10 min) without any equipment. (C) 2009 Elsevier Inc. All rights reserved.
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