Journal
ANALYTICAL BIOCHEMISTRY
Volume 392, Issue 2, Pages 139-144Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ab.2009.05.042
Keywords
Genomic DNA; Fluidic force discrimination (FFD); Biosensor; Magnetic bead; Fragmentation by sonication; Bacillus anthracis; Bacillus thuringiensis
Funding
- Office of Naval Research
- Nova Research, Inc.
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Herein, we describe the direct detection of genomic DNA using fluidic force discrimination (FFD) assays. Starting with extracted bacterial DNA, samples are fragmented by restriction enzymes or sonication, then thermocycled in the presence of blocking and labeling sequences, and finally detected with microbead-based FFD assays. Both strain and species identification of extracted Bacillus DNA have been demonstrated in <30 min, without amplification (e.g., PCR). Femtomolar assays can be achieved with this rapid and simple procedure. (C) 2009 Elsevier Inc. All rights reserved.
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