Journal
ANALYTICAL BIOCHEMISTRY
Volume 384, Issue 2, Pages 254-262Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ab.2008.09.050
Keywords
IRDye 800CW; 2-Deoxyglucose; Near infrared; Molecular imaging; Immunofluorescent assay; Fluorescence microscopy; Whole animal imaging
Funding
- NCI NIH HHS [R01 CA106584-04, R01 CA106584-01A1, R01 CA106584-02, R01 CA106584-03, R01 CA106584] Funding Source: Medline
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Malignant neoplasms exhibit an elevated rate of glycolysis Over normal cells. This characteristic can be exploited for optical imaging of tumors in mice. A near-infrared fluorophore, IRDye 800CW, emission maximum 794 nm, was Conjugated to 2-deoxyglucose (2-DG). An immunofluorescent cell-based assay Was used to evaluate specificity and sensitivity of the Conjugate in Cultured cell monolayers. Dose-dependent uptake was established with increasing concentrations of IRDye 800CW 2-DG for epithelial and Prostate carcinomas. IRDye 800CW 2-DG was specifically blocked by an antibody against GLUT1 glucose transporter, and by excess unlabeled 2-DG or D-glucose. Signal was increased by a phorbol ester activator Of glucose transport. Fluorescence microscopy data confirmed localization of the Conjugate in the cytoplasm. Subsequent ill Vivo Studies optimized dose, clearance, and timing for Signal Capture in nude mouse xenografts. In all cases, tumors were clearly imaged with good signal-to-noise characteristics. These data indicate that IRDye 800CW 2-DG is a broadly applicable optical imaging agent for in vivo imaging of neoplasms in mice. (C) 2008 Elsevier Inc. All rights reserved.
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