Journal
JOURNAL OF PROTEOME RESEARCH
Volume 6, Issue 8, Pages 3224-3231Publisher
AMER CHEMICAL SOC
DOI: 10.1021/pr0701456
Keywords
S-nitrosylation; proteomics; mass spectrometry; post-translational modifications; nitric oxide
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S-nitrosylation is emerging as an important signaling mechanism that regulates a broad range of cellular functions. The recognition of Cysteine residues that undergo S-nitrosylation is crucial to elucidate how this modification modulates protein activity. We report here a novel strategy, defined His-tag switch, which allows the purification and identification of S-nitrosylated proteins and the unambiguous localization of the modified cysteine residues by mass spectrometry analysis.
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