Journal
ANALYTICAL BIOCHEMISTRY
Volume 384, Issue 1, Pages 27-33Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ab.2008.09.034
Keywords
Cresols; Feces; HPLC; Human; Phenol; Urine; Vacuum microdistillation
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In this article, we describe a simple, sensitive, accurate, and repeatable method for the measurement of phenol and p-cresol (4-methylphenol) in human urine and feces. We examined a number of parameters to identify all optimal extraction protocol. Purification of sample extracts was achieved by low-temperature vacuum microdistillation. Separation was achieved in approximately 15 min by high-performance liquid chromatography (HPLC) with quantification by fluorescence at 284/310 nm. Limits of detection for phenol were 2 ng/ml for urine and 20 ng/g for feces, and those for p-cresol were 10 ng/ml for urine and 100 ng/g for feces. For comparison, approximate mean values for urine are 3 mu g/ml for phenol and 30 mu g/ml for p-cresol, and those for feces are 1 mu g/g for phenol and 50 mu g/g for p-cresol. An experienced analyst can process 60 samples each day using this method. Crown Copyright (C) 2008 Published by Elsevier Inc. All rights reserved.
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