Journal
ANALYTICAL BIOCHEMISTRY
Volume 386, Issue 2, Pages 285-287Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ab.2008.12.009
Keywords
-
Funding
- State of Colorado
- University of Colorado Technology Transfer Office
- National Institutes of Health [R21 CA 127884]
- US Department of Education
- Teets Family Endowed Doctoral Fellowship in Nanotechnology
Ask authors/readers for more resources
Polymerization-based signal amplification offers sensitive visualization of biotinylated biomolecules functionalized to glass microarrays in a manner suitable for point-of-care use. Here we report using this method for visual detection of multiplexed nucleic acid hybridizations from complex media and develop an application toward point Mutation detection and single nucleotide polymorphism (SNP) typing, Primer extension reactions were employed to label selectively and universally all complementary surface DNA hybrids with photoinitiators, permitting simultaneous and dynamic photopolymerization from positive sites to 0.5-nM target concentrations. Dramatic improvements in signal ratios between complementary and mismatched hybrids enabled visual discrimination of single base differences in KRAS codon-12 biomarkers. (C) 2008 Elsevier Inc. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available