Journal
ANALYTICAL BIOCHEMISTRY
Volume 388, Issue 2, Pages 331-338Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ab.2009.02.035
Keywords
Protein L; Peptostreptococcus; scFv; Antibody; Purification; Antibody fragment
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Recombinant antibody fragments consisting of variable domains can be easily produced in various host cells, but there is no universal system that call be used to purify and detect them in the free form or complexed with their antigen. Protein L (PpL) is a cell wall protein isolated from Peptostreptococcus magnus, which has been reported to interact with the V-KAPPA chain of some, but not all, antibodies. Here we grafted the V-KAPPA framework region 1 (FR1) sequence of a high-affinity PpL-binding antibody onto single-chain antibody fragments (scFvs), which have no reactivity with PpL This substitution made it possible to Purify and detect scFvs using PpL conjugates. It did not hinder scFv folding and expression in recombinant bacteria, and it did not interfere with their antigen-binding function. We also identified residue 12 as being potentially able to alter PpL blinding. This study, therefore, suggests a way of engineering a PpL-binding site on any scFv Without interfering with its function. This could provide a universally applicable method both for the rapid purification of functional recombinant antibody fragments and for their detection even when complexed with their antigen without requiring fusion to in epitope Flag. (c) 2009 Elsevier Inc. All rights reserved.
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