A solution for stripping antibodies from polyvinylidene fluoride immunoblots for multiple reprobing

Available protocols for stripping antibodies from immunoblots involve the use of sodium dodecyl sulfate (SDS) or low-pH buffers. SDS was shown to remove transferred proteins from membranes, and low-pH buffer was shown to inefficiently strip off antibodies. A solution containing 6 M guanidine hydrochloride, 0.2% nondenaturing detergent, and a reducing agent can rapidly strip off tightly bound antibodies from) aged polyvinylidene fluoride (PVDF) immunoblots at room temperature Without removing significant amounts of transferred protein. (C) 2009 Elsevier Inc. All rights reserved.