Journal
INTERNATIONAL JOURNAL FOR PARASITOLOGY
Volume 37, Issue 10, Pages 1107-1115Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.ijpara.2007.02.011
Keywords
helminth parasite; transfection; promoter; terminator; electroporation
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Funding
- NIAID NIH HHS [N01-AI-30026] Funding Source: Medline
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We sought to investigate the efficacy of electroporation for the introduction of plasmid-based DNA constructs into Schistosoma mansoni, and expanded our study to examine parameters governing transgene expression, including requirements of a 5' and 3' flanking sequence, as well as parasite developmental effects on transgene expression. We used luciferase as a reporter gene for this application. Our data show that electroporation allows the transfection of immature schistosomes, and defines 5' promoter sequence from the schistosome actin gene (SmAct1.1), coupled promiscuously with various 3' terminator sequences, as a powerful promoter of transgene expression in growing, but not early non-growing, schistosomula. The methodology described herein will facilitate ectopic expression of genes of interest in schistosomes. (c) 2007 Australian Society for Parasitology Inc. Published by Elsevier Ltd. All rights reserved.
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