Journal
ANALYTICAL BIOCHEMISTRY
Volume 373, Issue 2, Pages 253-262Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ab.2007.10.026
Keywords
DNA purification; RNA purification; nucleic acid separation; silica; influenza detection; solid-phase extraction
Funding
- NIAID NIH HHS [R01 AI049541, R01 AI49541, R21 AI049541-04, R01 AI049541-04A2, R01 AI049541-05, R01 AI049541-03] Funding Source: Medline
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We report nucleic acid (NA) adsorption isotherms and elution profiles for silica surfaces and use these to design a miniaturized NA purification unit based on solid-pliase extraction with silica beads. The procedure is based on a pressure drop equation for flow through a packed bed and allows estimation of key design parameters such as channel dimensions, liquid flow rates, sample volume, and amount of silica needed. The usefulness of this design procedure is demonstrated by applying it to a column-based NA purification device for influenza detection for a case study of Madin-Darby canine kidney cells infected with influenza A virus. (C) 2007 Elsevier Inc. All rights reserved.
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