Journal
ANALYTICAL BIOCHEMISTRY
Volume 383, Issue 2, Pages 194-199Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ab.2008.08.039
Keywords
Cholesterol; Cholesterol oxidase (ChOx); Polyaniline (PANI); Carbon nanotubes (CNTs); Biosensor
Funding
- Department of Science and Technology (DST), Government of India [DST/TSG/ME/2007/06]
- India-Japan Project [DST/INT/JAP/P-21/07]
- Department of Biotechnology, Government of India [DBT/GAP070832]
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Nanocomposite film composed of polyaniline (PANI) and multiwalled carbon nanotubes (MWCNT), prepared electrophoretically onto indium tin oxide (ITO)-coated glass plate, was used for covalent immobilization of cholesterol oxidase (ChOx) via N-ethyl-N'-(3-dimethylaminopropyl) carbodiimide (EDC) and N-hydroxysuccinimide (NHS) chemistry. Results of linear sweep voltammetric measurements reveal that ChOx/PANI-MWCNT/ITO bioelectrode can detect cholesterol in the range of 1.29 to 12.93 mM with high sensitivity of 6800 nA mM(-1) and a fast response time of 10 s. Photometric studies for ChOx/PANI-MWCNT/ITO bioelectrode indicate that it is thermally stable LIP to 45 degrees C and has a shelf life of approximately 12 weeks when stored at 4 degrees C. The results of these studies have implications for the application of this interesting matrix (PANI-MWCNT) toward the development of other biosensors. (C) 2008 Elsevier Inc. All rights reserved.
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