Journal
ANALYTICAL BIOCHEMISTRY
Volume 378, Issue 2, Pages 166-170Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ab.2008.03.054
Keywords
BODIPY; fluorophore; imaging; labeling; RNA; ribozyme; Tetrahymena
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As inexpensive and readily available fluorophores for 3' and 5' end labeling of RNA molecules, symmetrical BODIPY (boron dipyrromethene: 4,4-difluoro-4-bora-3a,4a-diaza-s-indacene) derivatives having a primary amino group were designed, and their facile synthetic route was established. Novel BODIPY derivatives exhibited photophysical properties comparable to commercially available BODIPY FL EDA (4,4-difluoro-5,7-dimethyl-4-bora-3 a,4a-diaza-s-indacene-3-propionyl ethylenediamine). To confirm utility of new derivatives, specific labeling of the 3' and 5' ends of in vitro transcribed RNAs was carried out. Furthermore, the 3 ' end of the 5 ' fragment of the bimolecular Tetrahymena ribozyme was labeled, and its catalytic activity was investigated. (C) 2008 Elsevier Inc. All rights reserved.
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