Journal
NATURE METHODS
Volume 4, Issue 8, Pages 633-636Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH1065
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We describe a GFP- based RNA reporter system ( gimel(N)- GFP) to visualize RNA molecules in live mammalian cells. It consists of GFP fused to an arginine- rich peptide derived from the phage gimel N protein, gimel(N22), which binds a unique minimal RNA motif and can be used to tag any RNA molecule. gimel(N)- GFP uses a small and easy to engineer RNA tag, reducing the likelihood of perturbing the function of the tagged RNA molecule.
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