Journal
ELECTROANALYSIS
Volume 19, Issue 15, Pages 1591-1596Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/elan.200703888
Keywords
NADH; Ti-MCM-41; electrocatalytic; sensors; ADH; ethanol
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Titanium-containing MCM-41 (Ti-MCM-41) modified glassy carbon electrode (GCE) can exhibit an excellent electrocatalytic activity towards the oxidation of P-Nicotinamide adenine dinucleotide (NADH). A dramatic decrease in the overvoltage of NADH oxidation reaction is observed at 0.28 V vs. SCE. The application in the amperometric biosensing of ethanol using alcohol dehydrogenase enzyme (ADH) also has been demonstrated with this material. The proposed sensor shows a highly sensitivity, an acceptable reproducibility and a good stability. The linear range of ethanol is 25 - 1.000 mu M and the detection limit is 8.0 mu M. Ti-MCM-41 modified electrode not only can be used to detect the concentration of NADH in biochemical reaction, but also as the potential matrix for the construction of dehydrogenases sensor.
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