Use of a graphite-polyurethane composite electrode for electroanalytical determination of indole-3-acetic acid in soil samples

A new electroanalytical methodology was developed for the quantification of the phytohormone indole-3-acetic acid (IAA), using a graphite-polyurethane composite electrode (GPU) and the square wave voltammetry (SWV), in 0.1 mol L-1 phosphoric acid solution (PH 1.6). Analytical curves were constructed under optimized conditions (f= 100 s(-1), a= 50 mV, Delta E-i= 5 mV) and the reached detection and quantification limits were 26 mu g L-1 and 0.2 mg L-1, respectively. The developed methodology is simple and accurate for the routine determination of IAA. In order to verify the application of the electroanalytical methodology in fortified soil samples without previous treatment, an IAA assay was performed without serious interferences of the soil constituents. (c) 2007 Elsevier B.V. All rights reserved.