Journal
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
Volume 407, Issue 5, Pages 1505-1512Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-014-8355-x
Keywords
Artificial sweeteners; Steviol glycosides; Stevia rebaudiana; Tandem mass spectrometry; Liquid chromatography
Funding
- Polish National Science Centre grant [2012/07/N/ST4/01834]
- PSEZ (Pomeranian Special Economic Zone Ltd.)
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The method for the determination of acesulfame-K, saccharine, cyclamate, aspartame, sucralose, alitame, neohesperidin dihydrochalcone, neotame and five common steviol glycosides (rebaudioside A, rebaudioside C, steviol, steviolbioside and stevioside) in soft and alcoholic beverages was developed using high-performance liquid chromatography and tandem mass spectrometry with electrospray ionisation (HPLC-ESI-MS/MS). To the best of our knowledge, this is the first work that presents an HPLC-ESI-MS/MS method which allows for the simultaneous determination of all EU-authorised high-potency sweeteners (thaumatin being the only exception) in one analytical run. The minimalistic sample preparation procedure consisted of only two operations; dilution and centrifugation. Linearity, limits of detection and quantitation, repeatability, and trueness of the method were evaluated. The obtained recoveries at three tested concentration levels varied from 97.0 to 105.7 %, with relative standard deviations lower than 4.1 %. The proposed method was successfully applied for the determination of sweeteners in 24 samples of different soft and alcoholic drinks.
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