Journal
BIOTECHNOLOGY LETTERS
Volume 29, Issue 8, Pages 1249-1253Publisher
SPRINGER
DOI: 10.1007/s10529-007-9376-8
Keywords
cytokine; delivery systems; protein expression; synthetic gene; sec pathway
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A novel expression vector (pLR) driven by hup promoter and Bifidobacterium beta-galactosidase signal peptide was constructed. The pLR vector was used for the expression of the optimized human IL-10 synthetic gene in Escherichia coli and Bifidobacterium longum. In both microorganisms, rhIL-10 was in a soluble form in total extract cells. The recombinant hIL-10 was partially processed in E. coli, whereas in Bifidobacterium all rhIL-10 was found in the mature form.
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