Journal
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
Volume 407, Issue 4, Pages 1261-1265Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-014-8329-z
Keywords
Dimethyl phthalate (DMP); Biotin-streptavidin-amplified system; Real-time immuno-PCR; Beverage and drinking water samples
Funding
- National Natural Science Foundation of China [21177082]
- Science and Technology Commission of Shanghai Municipality in China (Key Project of Fundamental Research) [09JC1407600]
- Shanghai Jiao Tong University Science and Technology Innovation special fund development projects
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Dimethyl phthalate (DMP), one family of the phthalic acid diesters (PAEs), is an increasing widely used plasticizer. A sensitive and high-throughput direct competitive biotin-streptavidin-amplified system based on real-time immune-PCR (BA-rt-IPCR) techniques was developed for detecting DMP in beverage and drinking water samples. In our assay, we selected dimethyl 4-aminophthalate as the optimal DMP hapten to prepare high titer of rabbit polyclonal anti-DMP antibodies (pAb-DMP). Under the optimized conditions, the proposed method was used to detect DMP with a linearity range from 10 to 100 ng L-1, and the limit of detection (LOD) was 1.98 pg L-1. Finally, the results about DMP in beverage and drinking water samples were consistent with those using gas chromatography-mass spectrometry (GC-MS), which proved that the proposed immunoassay for detecting DMP in the environment was accurate, reliably rapid, and receptive.
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