Journal
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
Volume 407, Issue 3, Pages 669-680Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-014-8127-7
Keywords
Metabolomics; Gas chromatography-mass spectrometry; Liquid chromatography-mass spectrometry; Capillary electrophoresis-mass spectrometry; Microbial metabolomics
Funding
- National Basic Research Program of the State Ministry of Science and Technology of China [2007CB707800]
- Knowledge Innovation Program of the Chinese Academy of Sciences, the Foundation [21175132]
- Creative Research Group Project [21321064]
- National Natural Science Foundation of China [81372695]
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Metabolomics is an omics technique aiming at qualitatively and quantitatively describing a metabolome by various analytical platforms. It is an indispensable component of modern systems biology. Microbial metabolomics can be roughly classified as metabolic footprint analysis and metabolic fingerprint analysis depending on the analyte origins. Both of them have been beneficial to microbiological research for different reasons. Mass spectrometry and nuclear magnetic resonance spectroscopy techniques are popular analytical strategies prevailing in the metabolomics field. In this review, chromatography-mass-spectrometry-based microbial metabolomic analysis steps are summarized, including sample collection, metabolite extraction, instrument analysis, and data analysis. Moreover, their applications in some representative fields are discussed as examples. The aim of this review is to present briefly recent technical advances in mass-spectrometry-based analysis, and to highlight the value of modern applications of microbial metabolomics.
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