Journal
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
Volume 405, Issue 15, Pages 5119-5131Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-013-6732-5
Keywords
Mass spectrometry; Host-pathogen interactions; Liquid chromatography
Funding
- ERA-NET PathoGenoMics [0315442C]
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Infections with Chlamydia pneumoniae cause several respiratory diseases, such as community-acquired pneumonia, bronchitis or sinusitis. Here, we present an integrated non-targeted metabolomics analysis applying ultra-high-resolution mass spectrometry and ultra-performance liquid chromatography mass spectrometry to determine metabolite alterations in C. pneumoniae-infected HEp-2 cells. Most important permutations are elaborated using uni- and multivariate statistical analysis, logD retention time regression and mass defect-based network analysis. Classes of metabolites showing high variations upon infection are lipids, carbohydrates and amino acids. Moreover, we observed several non-annotated compounds as predominantly abundant after infection, which are promising biomarker candidates for drug-target and diagnostic research.
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