Journal
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
Volume 405, Issue 14, Pages 4719-4728Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-013-6899-9
Keywords
MALDI; Mass spectrometry imaging; Lipid; Protein; Tandem mass spectrometry
Funding
- EPSRC through the PSIBS Doctoral Training Centre at the University of Birmingham [EP/F50053X/1]
- EPSRC [EP/F50053X/1] Funding Source: UKRI
Ask authors/readers for more resources
Matrix-assisted laser desorption ionization mass spectrometry imaging (MALDI MSI) techniques are continually being assessed with a view to improving the quality of information obtained from a given sample. A single tissue section will typically only be analyzed once by MALDI MSI and is then either used for histological staining or discarded. In this study, we explore the idea of repeat analysis of a single tissue section by MALDI MSI as a route toward improving sensitivity, structural characterization, and diversity of detected analyte classes. Repeat analysis of a single tissue section from a fresh frozen mouse brain is investigated with both alpha-cyano-4-hydroxycinnamic acid (CHCA) and para-nitroaniline (PNA). Repeat analysis is then applied to the acquisition of MALDI MSI and MALDI tandem mass spectrometry imaging employing collision induced dissociation (MS/MS imaging employing CID) from a formalin-fixed mouse brain section. Finally, both lipid and protein data are acquired from the same tissue section via repeat analysis utilizing CHCA, sinapinic acid (SA), and a tissue wash step. PNA was found to outperform CHCA as a matrix for repeat analysis; multiple lipids were identified using MS/MS imaging; both lipid and protein images were successfully acquired from a single tissue section.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available