Journal
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
Volume 406, Issue 3, Pages 695-704Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-013-7496-7
Keywords
Foods/beverages; Mass spectrometry imaging; Fungi; Secondary metabolites; MALDI; High resolution
Funding
- Hessian Ministry of Science and Art (LOEWE Research Focus 'integrative fungal research')
- Deutsche Forschungsgemeinschaft (DFG) [Sp 314/13-1]
Ask authors/readers for more resources
Fungal secondary metabolites in both fruiting bodies and pellets from submerged cultures of basidiomycetes were analyzed by atmospheric pressure matrix-assisted laser desorption/ionization-mass spectrometry imaging at a lateral resolution of 15 mu m, a mass resolution of 140,000 at m/z 200 and a mass accuracy of better than 2 ppm. The striatals A, B, C, and D, and a number of erinacine type metabolites were detected in the basidiomycetes Cyathus striatus and Hericium erinaceus, respectively. The two fungi were selected as model species, as they are well-known for efficient production of terpenoid secondary metabolites with interesting biological activities, e.g., antibacterial, fungicidal, cytotoxic properties, and stimulating effects on nerve growth factor synthesis. The localization of metabolites revealed a mostly homogeneous distribution of the striatals in the pellets of C. striatus, while a concentration gradient, increasing to the center, was observed in the pellets of H. erinaceus. A mostly homogeneous distribution of metabolites was also found in the fruiting body of H. erinaceus.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available