Journal
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
Volume 405, Issue 20, Pages 6511-6517Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-013-7089-5
Keywords
Comprehensive two-dimensional liquid chromatography; Shift gradient; Peak distribution; Chinese herbal medicine
Funding
- China Scholarship Council (CSC)
- German Academic Exchange Service (DAAD)
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Comprehensive two-dimensional liquid chromatography (LC x LC) has received much attention because it offers much higher peak capacities than separation in a single dimension. The advantageous peak capacity makes it attractive for the separation of complex samples. Various gradient methods have been used in LC x LC systems. The use of continuous shift gradient is advantageous because it combines the peak compression effect of full gradient mode and the tailed gradient program in parallel gradient mode. Here, a comparison of LC x LC analysis of Chinese herbal medicine with full gradient mode and shift gradient mode in the second dimension was performed. A correlation between the first and second dimensions was found in full gradient mode, and this was significantly reduced with shift gradient mode. The orthogonality increased by 43.7 %. The effective peak distribution area increased significantly, which produced better separation.
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