Journal
JOURNAL OF MASS SPECTROMETRY
Volume 42, Issue 8, Pages 1093-1098Publisher
WILEY
DOI: 10.1002/jms.1245
Keywords
ion mobility MS; lipid imaging; brain tissue; phosphatidylcholines; cerebrosides
Funding
- Intramural NIH HHS [Z99 DA999999] Funding Source: Medline
- NIDA NIH HHS [N44DA-3-7727] Funding Source: Medline
- PHS HHS [HHSN271200677563C, HHSN271200677593C] Funding Source: Medline
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While maintaining anatomical integrity, matrix assisted laser desorption/ionization mass spectrometry (MALDI-MS) has allowed researchers to directly probe tissue, map the distribution of analytes and elucidate molecular structure with minimal preparation. MALDI-ion mobility (IM)-orthogonal time-of-flight mass spectrometry (oTOFMS) provides an advantage by initially separating different classes of biomolecules such as lipids, peptides, and nucleotides by their IM drift times prior to mass analysis. In the present work the distribution of phosphatidlycholine and cerebroside species was mapped from 16 mu m thick coronal rat brain sections using MALDI-IM-oTOFMS. Furthermore, the use of gold nanoparticles as a matrix enables detection of cerebrosides, which although highly concentrated in brain tissue, are not easily observed as positive ions because of intense signals from lipids such as phosphatidlycholines and sphingomyelins. Copyright (c) 2007 John Wiley & Sons, Ltd.
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