Journal
CYTOMETRY PART A
Volume 71A, Issue 8, Pages 563-575Publisher
WILEY
DOI: 10.1002/cyto.a.20404
Keywords
particle detection; machine learning; Haar features; signal-to-noise ratio
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Funding
- NLM NIH HHS [R01 LM008696] Funding Source: Medline
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Clathrin-coated pits play an important role in removing proteins and lipids from the plasma membrane and transporting them to the endosomal compartment. It is, however, still unclear whether there exist hot spots for the formation of Clathrin-coated pits or the pits and arrays formed randomly on the plasma membrane. To answer this question, first of all, many hundreds of individual pits need to be detected accurately and separated in live-cell microscope movies to capture and monitor how pits and vesicles were formed. Because of the noisy background and the low contrast of the live-cell movies, the existing image analysis methods, such as single threshold, edge detection, and morphological operation, cannot be used. Thus, this paper proposes a machine learning method, which is based on Haar features, to detect the particle's position. Results show that this method can successfully detect most of particles in the image. In order to get the accurate boundaries of these particles, several post-processing methods are applied and signal-to-noise ratio analysis is also performed to rule out the weak Spots. (C) 2007 International Society for Analytical Cytology
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