Journal
ACTA BIOCHIMICA ET BIOPHYSICA SINICA
Volume 39, Issue 8, Pages 549-559Publisher
BLACKWELL PUBLISHING
DOI: 10.1111/j.1745-7270.2007.00320.x
Keywords
FTIR; protein structure; protein dynamic
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Infrared spectroscopy is one of the oldest and well established experimental techniques for the analysis of secondary structure of polypeptides and proteins. It is convenient, non-destructive, requires less sample preparation, and can be used under a wide variety of conditions. This review introduces the recent developments in Fourier transform infrared (FTIR) spectroscopy technique and its applications to protein structural studies. The experimental skills, data analysis, and correlations between the FTIR spectroscopic bands and protein secondary structure components are discussed. The applications of FTIR to the secondary structure analysis, conformational changes, structural dynamics and stability studies of proteins are also discussed.
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