Journal
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
Volume 404, Issue 3, Pages 711-720Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-012-6092-6
Keywords
Protein phosphatase; Microcystin; Artificial neural network; Influence of organic solvents
Funding
- Romania-France bilateral grant Brancusi [487/2011]
- Romanian Ministry of Labour, Family and Social Protection [POSDRU/6/1.5/S/19]
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The inhibition characteristics of three different protein phosphatases by three microcystin (MC) variants-LR, YR, and RR-were studied. The corresponding K (I) for each enzyme-MC couple was calculated. The toxicity of MC varies in the following order: MC-LR > MC-YR > MC-RR. The sensitivity of the enzymes increased in the following order: mutant PP2A < mutant PP1 < natural PP2A. The best limit of detection obtained was 21.2 pM MC-LR using the most sensible enzyme. Methanol, ethanol, and acetonitrile up to 2 % (v/v) may be used in inhibition measurements. An artificial neural network (ANN) was used to discriminate two MC variants-LR and YR-using the differences in inhibition percentages measured with mutant PP1 and natural PP2A. The ANN is able to analyze mixtures with concentrations ranging from 8 to 98 pM MC-LR and 31 to 373 pM MC-YR.
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