4.7 Article

Determination of bisphenol A and bisphenol B in canned seafood combining QuEChERS extraction with dispersive liquid-liquid microextraction followed by gas chromatography-mass spectrometry

Journal

ANALYTICAL AND BIOANALYTICAL CHEMISTRY
Volume 404, Issue 8, Pages 2453-2463

Publisher

SPRINGER HEIDELBERG
DOI: 10.1007/s00216-012-6389-5

Keywords

DLLME (dispersive liquid-liquid microextraction); BPA (bisphenol A); BPB (bisphenol B); Canned food; Gas chromatography-mass spectrometry (GC-MS)

Funding

  1. FCT [PTDC/AGR-ALI/101583/2008]
  2. COMPETE FSE/FEDER
  3. Fundo Social Europeu e Fundo Nacional MCTES
  4. Fundação para a Ciência e a Tecnologia [PTDC/AGR-ALI/101583/2008] Funding Source: FCT

Ask authors/readers for more resources

A new simple and reliable method combining an acetonitrile partitioning extractive procedure followed by dispersive solid-phase cleanup (QuEChERS) with dispersive liquid-liquid microextraction (DLLME) and further gas chromatography mass spectrometry analysis was developed for the simultaneous determination of bisphenol A (BPA) and bisphenol B (BPB) in canned seafood samples. Besides the great enrichment factor provided, the final DLLME extractive step was designed in order to allow the simultaneous acetylation of the compounds required for their gas chromatographic analysis. Tetrachloroethylene was used as extractive solvent, while the acetonitrile extract obtained from QuEChERS was used as dispersive solvent, and anhydride acetic as derivatizing reagent. The main factors influencing QuEChERS and DLLME efficiency including nature of QuEChERS dispersive-SPE sorbents, amount of DLLME extractive and dispersive solvents and nature and amount of derivatizing reagent were evaluated. DLLME procedure provides an effective enrichment of the extract, allowing the required sensitivity even using a single quadropole MS as detector. The optimized method showed to be accurate (> 68 % recovery), reproducible (< 21 % relative standard deviation) and sensitive for the target analytes (method detection limits of 0.2 mu g/kg for BPA and 0.4 mu g/kg for BPB). The screening of several canned seafood samples commercialized in Portugal (total = 47) revealed the presence of BPA in more than 83 % of the samples with levels ranging from 1.0 to 99.9 mu g/kg, while BPB was found in only one sample at a level of 21.8 mu g/kg.

Authors

I am an author on this paper
Click your name to claim this paper and add it to your profile.

Reviews

Primary Rating

4.7
Not enough ratings

Secondary Ratings

Novelty
-
Significance
-
Scientific rigor
-
Rate this paper

Recommended

No Data Available
No Data Available