Journal
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
Volume 41, Issue 3, Pages 354-357Publisher
ELSEVIER
DOI: 10.1016/j.ijbiomac.2007.04.002
Keywords
erythropoietin; isoelectric profiles; serum/urine comparison
Ask authors/readers for more resources
By adding a step of immunoaffinity to the method we had previously developed for analysing erythropoietin (EPO) in urine, we were able to study the isoelectric profiles of this hormone in human serum samples. This method was sensitive enough to investigate samples presenting physiological levels of this hormone. Comparison with the corresponding profiles in urine showed that natural EPO was systematically more acidic in urine. The acidification process, which was not patent in the non-human primate Cynomolgus macaque, clearly also affected recombinant EPO when injected into humans. This process was unrelated to any enzymatic activity in urine since the incubation of natural or recombinant EPO in urine induced no transformation of their isoclectric profiles. The nature and mechanism of the structural modifications occurring during the renal handling of this hormone remain to be investigated. (c) 2007 Published by Elsevier B.V.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available