Journal
MOLECULAR AND CELLULAR BIOLOGY
Volume 27, Issue 15, Pages 5587-5596Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/MCB.01883-06
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Funding
- NCI NIH HHS [CA16038, P01 CA016038] Funding Source: Medline
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Approximately 30% of human tumors examined for mutations in polymerase beta (poll 0) appear to express pol beta variant proteins (D. Starcevic, S. Dalai, and J. B. Sweasy, Cell Cycle 3:998-1001, 2004). Many of these variants result from a single amino acid substitution. We have previously shown that the K289M and 1260M colon and prostate cancer variants, respectively, induce cellular transformation most likely due to sequence-specific mutator activity (S. Dalai et all., Biochemistry 44:15664-15673, 2005; T. Lang et al., Proc. Natl. Acad. Sci. USA 101:6074-6079, 2004; J. B. Sweasy et al., Proc. Natl. Acad. Sci. USA 102:14350-14355, 2005). In the work described here, we show that the E295K gastric carcinoma pol 0 variant acts in a dominant-negative manner by interfering with base excision repair. This leads to an increase in sister chromatid exchanges. Expression of the E295K variant also induces cellular transformation. Our data suggest that unfilled gaps are channeled into a homology-directed repair pathway that could lead to genomic instability. The results indicate that base excision repair is critical for maintaining genome stability and could therefore be a tumor suppressor mechanism.
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