Journal
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
Volume 404, Issue 3, Pages 909-917Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-012-6159-4
Keywords
Dispersive liquid-liquid microextraction; Sequential injection analysis; Sample treatment automation; Fatty alcohols; Rhodamine B
Funding
- Ministerio de Ciencia y Tecnologia (Spain) [CTQ2010-15541]
- Spanish National Research Council (CSIC)
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A new approach for the integration of various analytical steps inside a syringe (Lab in a Syringe) is presented. Fully automated dispersive liquid-liquid microextraction with integrated spectrophotometric detection is carried out in-syringe using a very simple instrumental setup. The lighter-than-water organic droplets released in the extraction step accumulate at the head of the syringe, where two optical fibers are placed on both sides of the syringe, facing each other and enabling the in situ quantification of the extracted compounds. By this, monitoring of the progressively accumulating droplet in the head of the syringe was further possible. In this first report, the developed instrumental setup has been applied to the determination of the dye rhodamine B in water samples and soft drinks. The main parameters influencing the extraction such as the selection of the extractant and disperser solvents, extractant/disperser and organic/water phase ratios, pH of the aqueous phase, extraction flow rates, and extraction time were investigated. Under the selected conditions, rhodamine B was quantified in a working range of 0.023-2 mg L-1 with a limit of detection of 0.007 mg L-1. Good repeatability values of up to 3.2 % (RSD) were obtained for ten consecutive extractions. The enrichment factor for a 1 mg L-1 rhodamine B standard was 23, and up to 51 extractions were accomplished in 1 h.
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