Journal
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
Volume 404, Issue 10, Pages 3061-3069Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-012-6408-6
Keywords
Microfluidic chip; Marine microalgae; Motility; Toxicity assessment; Chemostatic module
Funding
- National Natural Science Foundation of China [41006071]
- China Post-doctoral Science Foundation [20100481241]
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A polydimethylsiloxane microfluidic chip has been developed for the estimation of toxic heavy metals based on measurement of mobility of marine microalgae. The chip is mainly composed of an upstream concentration gradient generator and a downstream perfusion-based chemotatic module. The processes of toxic liquid dilution and diffusion, microalgal culturing, cell stimulation, and online screening can be integrated in this chip, which makes it an attractive approach to simplify toxicity testing procedures. The microalgal motility was adopted as a microfluidic bioassay signal and was evaluated as the percentage of motile cells, curvilinear velocity, average path velocity, and straight line velocity. Two mobile marine microalgae, Platymonas subcordiformis and Platymonas helgolandica var. tsingtaoensis, were confined in the chemotatic module and stimulated by the eight concentration gradients of Cu and Cd generated by the concentration gradient generator. In all cases, a toxic response was detected (i.e., a dose-related inhibition of motility was observed). Only 1.5 h was needed to predict EC50 values. Thus, the microfluidic chip developed was proved to be useful as a simple and rapid approach in heavy metal detection and might be expanded as a conventional test method in environmental toxicity assessment.
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