Journal
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
Volume 855, Issue 1, Pages 71-79Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jchromb.2007.05.002
Keywords
rice core collection; GC x GC; two-dimensional chromatography; gas chromatography; metabolic phenotyping
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We have developed a comprehensive method combining analytical techniques of one-dimensional (I D) and two-dimensional (GC x GC) gas chromatography-time-of-flight (TOF)-mass spectrometry. This method was applied to the metabolic phenotyping of natural variants in rice for the 68 world rice core collection (WRC) and two other varieties. Ten metabolites were selected as metabolite representatives, and the selected ion current of each metabolite peak obtained from both techniques were statistically compared. Our method of combining I D- and GC x GC-TOF/MS is useful for the metabolic phenotyping of natural variants in rice for further studies in breeding programs. (C) 2007 Elsevier B.V. All rights reserved.
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