Journal
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
Volume 405, Issue 6, Pages 1821-1831Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-012-6413-9
Keywords
Blue native polyacrylamide gel electrophoresis; Metabolic dysfunction; Metal toxicity; Enzymes; Functional proteomics; Diseases
Funding
- Laurentian University
- Industry Canada
- Natural Sciences and Engineering Research Council of Canada
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Gel electrophoresis is routinely used to separate and analyse macromolecules in biological systems. Although many of these electrophoretic techniques necessitate the denaturing of the analytes prior to their analysis, blue native polyacrylamide gel electrophoresis (BN-PAGE) permits the investigation of proteins/enzymes and their supramolecular structures such as the metabolon in native form. This attribute renders this analytical tool conducive to deciphering the metabolic perturbations invoked by metal toxicity. In this review, we elaborate on how BN-PAGE has led to the discovery of the dysfunctional metabolic pathways associated with disorders such as Alzheimer's disease, Parkinson's disease, and obesity that have been observed as a consequence of exposure to various metal toxicants.
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