4.7 Article

Chemoselective reduction-based fluorescence probe for detection of hydrogen sulfide in living cells

Journal

ANALYTICAL AND BIOANALYTICAL CHEMISTRY
Volume 404, Issue 6-7, Pages 1919-1923

Publisher

SPRINGER HEIDELBERG
DOI: 10.1007/s00216-012-6292-0

Keywords

Hydrogen sulfide; Fluorescence probes; Azides; Resorufamine

Funding

  1. National Natural Science Foundation of China [21072015]
  2. Program for New Century Excellent Talents in University [NCET-10-0203]
  3. Ministry of Education [BMU20110263]

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A selective and sensitive fluorescence probe for hydrogen sulfide (H2S) detection was synthesized and evaluated in PBS buffer and fetal bovine serum. The effect of pH on the probe was also studied. In addition, visualization of H2S in Hela cells was achieved using confocal laser scanning fluorescence microscopy.

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