4.7 Review

Affinity monolith chromatography: a review of principles and recent analytical applications

Journal

ANALYTICAL AND BIOANALYTICAL CHEMISTRY
Volume 405, Issue 7, Pages 2133-2145

Publisher

SPRINGER HEIDELBERG
DOI: 10.1007/s00216-012-6568-4

Keywords

Affinity monolith chromatography; Monolithic supports; Affinity chromatography; Bioaffinity chromatography; Immunoaffinity chromatography; Immobilized-metal-ion affinity chromatography; Dye-ligand affinity chromatography; Biointeraction chromatography

Funding

  1. National Institutes of Health [R01 GM044931]
  2. National Science Foundation (NSF) REU program
  3. NSF/EPSCoR program [EPS-1004094]
  4. Direct For Mathematical & Physical Scien
  5. Division Of Chemistry [1156560] Funding Source: National Science Foundation
  6. EPSCoR
  7. Office Of The Director [1004094] Funding Source: National Science Foundation

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Affinity monolith chromatography (AMC) is a type of liquid chromatography that uses a monolithic support and a biologically related binding agent as a stationary phase. AMC is a powerful method for the selective separation, analysis, or study of specific target compounds in a sample. This review discusses the basic principles of AMC and recent developments and applications of this method, with particular emphasis being given to work that has appeared in the last 5 years. Various materials that have been used to prepare columns for AMC are examined, including organic monoliths, silica monoliths, agarose monoliths, and cryogels. These supports have been used in AMC for formats that have ranged from traditional columns to disks, microcolumns, and capillaries. Many binding agents have also been employed in AMC, such as antibodies, enzymes, proteins, lectins, immobilized metal ions, and dyes. Some applications that have been reported with these binding agents in AMC are bioaffinity chromatography, immunoaffinity chromatography or immunoextraction, immobilized-metal-ion affinity chromatography, dye-ligand affinity chromatography, chiral separations, and biointeraction studies. Examples are presented from fields that include analytical chemistry, pharmaceutical analysis, clinical testing, and biotechnology. Current trends and possible directions in AMC are also discussed.

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