Journal
CELL RESEARCH
Volume 17, Issue 8, Pages 682-688Publisher
INST BIOCHEMISTRY & CELL BIOLOGY
DOI: 10.1038/cr.2007.61
Keywords
embryonic stem cells; inner cell mass; derivation; feeder cells; xeno-free; differentiation
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Human embryonic stem cells (hESC) not only hold great promise for the treatment of degenerative diseases but also provide a valuable tool for developmental studies. However, the clinical applications of hESC are at present limited by xeno-contamination during the in vitro derivation and propagation of these cells. In this review, we summarize the current methodologies for the derivation and the propagation of hESC in conditions that will eventually enable the generation of clinical-grade cells for future therapeutic applications.
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